Transcript abundance of jasmonate metabolic genes, and seed jasmonic acid (JA) and JA-isoleucine (JA-Ile) content.

<p>Jasmonate metabolism pathway in plants (A). Expression of probesets annotated as jasmonate metabolic genes in log₂ fold change during imbibition of dormant (D-12/D-0, D-24/D-0 and D-24/D-0) and after-ripened (AR-12/AR-0, AR-24/AR-0 and AR-24/AR-0) seeds as shown in the first column of the heat map, between dormant and after-ripened seeds in both dry and imbibed states (AR-0/D-0, AR-12/D-12 and AR-24/D-24) and between water and ABA imbibed after-ripened seeds (AR-24/AR-24+ABA) as shown in the second column in each heat map (B). Determination of the fold changes in expression of each probeset is as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056570#pone-0056570-g002" target="_blank">Figure 2</a>. Log₂ and linear scaled fold changes in expression of the probesets and the respective <i>P</i> values can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056570#pone.0056570.s003" target="_blank">Table S2</a>. JA and JA-Ile content of D and AR seeds in dry (0 days after imbibition [HAI]) and imbibed (12 and 24 HAI) states (C). Data are means of JA and JA-Ile measurements from three independent biological replicates ± SE. DAD, defender against cell death; LOX; lipoxygenase; 13-HPOT,13-hydroperoxylinolenic acid; AOS, allene oxide synthase; 12, 13-EOT, 12,13 epoxy-octadecatrienoic acid; AOC, allene oxide cyclase; cis-(+)-OPDA, cis-(+) -12-oxo phytodienoic acid; OPR3, 12-oxophytodienoate reductase; OPC-8∶0, 3-oxo-2-(2′-<i>Z</i>-pentenyl)-cyclopentane-1-octanoic acid; ACS, acyl-coenzyme A synthetase; ACX, acyl-coenzyme A oxidase; MFP, multifunctional protein; KAT, 3-ketoacyl coenzyme A thiolase; TEase; acyl-coenzyme A thioesterase; JAR, jasmonate resistant.