3xTg-AD/BDNF^+/− mice have reduced BDNF levels but no changes in BDNF-related signaling.

<p>(<b>A–B</b>) Western blot analysis on whole brain homogenates from homozygous 3xTg-AD mice reveals no significant difference in levels of mature BDNF as compared to wildtype controls (<i>n = </i>3). Levels of proBDNF trend toward an increase in 3xTg-AD mice, although this difference is not significant (<i>p</i> = 0.16). (<b>C</b>) 3xTg-AD/BDNF^+/− mice have a 43% reduction in BDNF protein by ELISA versus 3xTg-AD/BDNF^+/+ controls (<i>n = </i>4, <i>p</i> = 0.015). (<b>D</b>) Representative western blots for levels of various BDNF-related signaling proteins in the cerebral cortex of 3xTg-AD/BDNF^+/+ and 3xTg-AD/BDNF^+/− mice (<i>n = </i>6) are shown in alternating lanes. (<b>E</b>) Quantification of bands from <i>B</i> are normalized to GAPDH levels and shown as levels relative to 3xTg-AD/BDNF^+/+ controls. Data are presented as means ±SEM.