Deletion of <i>Kcne</i>2 results in a hyperpolarizing shift in HCN channel activation in VB neurons.

<p><b><i>A</i></b>, Representative families of current traces recoded in VB neurons from <i>Kcne2^+/+</i> and <i>Kcne2^−/−</i> mice; the voltage protocol is shown below. The tail currents from the same neurons are shown on an expanded time scale for comparison. Arrow indicates the trace elicited at −90 mV. <b><i>B</i></b>, Normalized tail currents (I_tail) are plotted as a function of voltage steps and are fit with the Boltzmann equation. V_1/2 values (in mV) were significantly shifted by the deletion to more hyperpolarized potentials. <b><i>C</i></b>, Comparison of I_h density in the two genotypes. *, P<0.01, one-way ANOVA, <i>vs. Kcne2^+/+</i>. <b><i>D</i></b>, Deletion of <i>Kcne</i>2 did not alter I_h reversal potential.