Isolation of single DRG neurons from <i>isl2b:EGFP</i> transgenic zebrafish.

<p><b><i>A</i></b>, Dissected central trunk region including the spinal cord and notochord from juvenile <i>isl2b:EGFP</i> fish (left panel). Enlarged dashed region shows EGFP-labeled DRG present in every trunk segment from the primary preparation (A-a and b). Note that the trunk segment had been exposed to enzymes at this point. <b><i>B</i></b>, Phase-contrast (B-a) and fluorescent (B-b) photomicrographs of acutely dissociated DRG neurons (circled) from the primary preparation. Scale bar in A (top) represent 0.1 mm and scale bar in B (bottom) represent 50 µm. <b><i>C</i></b>, Dot plots representing cell diameter of single dissociated DRG (filled circles) and R-B (open circles) neurons. Error bars represent standard deviation and statistical significant was determined using an unpaired t-test with Welch's correction.