Calcium sources for the GT-induced elevation of calpain 2 protein.

<p>(A) Representative immunoblots showing the changes of SBDP levels in cell extracts obtained from normal (Ctr), glutamate (Glu)-treated (0.5 mM for 6 h) with or without addition of D-APV (1 and 10 µM), and D-APV (1 and 10 µM)-treated groups. Bar chart summarizing the average densitometric quantification of immunoreactive bands of SBDPs under different conditions. (B) Representative immunoblots showing the changes of SBDP levels in cell extracts obtained from Ctr, Glu-treated (0.5 mM for 6 h) with or without addition of CNQX (1 and 10 µM), and CNQX (1 and 10 µM)-treated groups. Bar chart summarizing the average densitometric quantification of immunoreactive bands of SBDPs under different conditions. (C) Representative immunoblots showing the changes of SBDP levels in cell extracts obtained from Ctr, Glu-treated (0.5 mM for 6 h) with or without addition of dantrolene (Dan; 1 and 10 µM), and Dan (1 and 10 µM)-treated groups. Bar chart summarizing the average densitometric quantification of immunoreactive bands of SBDPs under different conditions. (D) Representative immunoblots showing the changes of SBDP levels in cell extracts obtained from Ctr, Glu-treated (0.5 mM for 6 h) with or without addition of xestospongin (Xes; 0.1 and 1 µM), and Xes (1 and 10 µM)-treated groups. Bar chart summarizing the average densitometric quantification of immunoreactive bands of SBDPs under different conditions. All data are normalized to Ctr. n = 6 for each group, ** <i>p</i><0.01, *** <i>p</i><0.001 <i>vs</i> control; ^##<i>p</i><0.01, ^###<i>p</i><0.001 <i>vs</i> Glu alone group, one-way ANOVA.