Generation of <i>hspb2</i> cardiac specific knockout mice.
- Takahiro Ishiwata (313155)
- András Orosz (313156)
- Xiaohui Wang (19899)
- Soumyajit Banerjee Mustafi (254807)
- Gregory W. Pratt (313157)
- Elisabeth S. Christians (313158)
- Sihem Boudina (71311)
- E. Dale Abel (71321)
- Ivor J. Benjamin (313159)
Publication date
February 2013
DOIAbstract
<p>(A). Schematic diagram showing the <i>hspb2</i> locus, targeting <i>hspb2</i> construct and the expected organization of the targeted <i>hspB2</i> locus before and after Cre-mediated recombination. Abbreviations: N:NcoI, E:EcoRI, Hp:HpaI, No:NotI, Neo:neomycin cassette, E1: exon1, E2: exon2. Green rectangles represent FRT recombinase recognition sequences and blue triangles depict the loxP sites (Cre recombinase recognition sites). (B) Representative Western blots showing expression levels of the CRYAB and HSPB2 in hearts (lane 1–6) and soleus (lane 7–12). Heart extracts do not contain HSPB2 in HSPB2cKO (lane 4–6) while its expression is maintained in the skeletal muscle, soleus (lane 10–12). GAPDH was used a loading control.</p
Add to ORKG