GFP reporter expression is induced in TNBS colitis model.

<p>(A) Representative white light and GFP images of whole colon from procollagen α1(I)-GFP reporter mice after one enema of TNBS versus ethanol or water control. Note increased GFP in distal colon of TNBS (and to a lesser extent, ethanol) treated mice. (B) Low power images of H&E and procollagen GFP activation obtained from control and TNBS treated mice colon (10X). (C) High power images demonstrate that GFP reporter expression is induced in submucosa and muscularis propria in colon of TNBS-treated mice. (D) High power images from immunostaining demonstrate colocalization of vimentin and GFP (white arrows) in submucosa and muscularis. Limited colocalization of α-SMA⁺ and GFP⁺ cells were detected in mucosa but not in submucosa and muscularis.