Effect of ADM on cell cycle in A2780 and OVCAR cells.

<p>Cells were starved for 36 hours with or without addition of ADM 100 nM, harvested after 0 (T1), 4 (T2), 8 (T3), and 24 hrs (T4), fixed, stained with propidium iodide and acquired using a cytofluorimeter. Data were analyzed as histogram plots. By plotting cell count versus propidium iodide (PI) fluorescence, the percentage of cells in each phase was evaluated. Data from A2780 and OVCAR-3 cells alone are shown as a percentage of the total G1+S+G2 (A–B). Cell death was also evaluated by analyzing the DNA fragmentation included in the sub-G_0/1 peak (C–D). The effect of ADM on cell cycle and on cell death is not detectable. This experiment was repeated twice with similar results.