GM-CSF transcriptional down-regulation and promoter chromatin resetting is independent of the cell cycle.

<p>(A–B) Asynchronised and synchronised EL-4 T cells were left unstimulated (NS) or stimulated with PI for 4 h (PI) then the stimulus withdrawn (WD) for the indicated times. GM-CSF mRNA levels relative to GAPDH were then determined by RT-qPCR (A) and promoter accessibility to MNase was determined by CHART-PCR (B). The mean and standard error of three independent experiments is shown. (C) GM-CSF mRNA levels relative to GAPDH were determined in EL-4 T cells stimulated with PI for 4 h (PI) and then the stimulus withdrawn (WD) for 4 h or 20 h, with or without nocodazole (NOC), as indicated. GM-CSF mRNA levels are shown relative to the PI sample which was set at 100%. (D) GM-CSF promoter accessibility to MNase was determined in EL-4 T cells treated as indicated. The mean and standard error of five independent experiments is shown (C–D).