Transcriptional down-regulation of the GM-CSF gene is dependent on the nuclear depletion of c-Rel.

<p>(A) Nuclear extracts from EL-4 T cells either left unstimulated (NS), stimulated with PI for 4 h (PI), the stimulus withdrawn for 4 h (WD) or the stimulus withdrawn in the presence of pentoxifylline (PTX) or lithium chloride (LiCl), were subjected to western analysis with the indicated antibodies. (B) GM-CSF mRNA levels relative to GAPDH were determined in cells treated as indicated. mRNA levels are shown relative to the 4 h PI sample which was set at 100%. The mean and standard error of three independent experiments is shown. (C–D) Nuclear extracts from EL-4 T cells either left unstimulated (NS), stimulated with PI for 4 h (PI), the stimulus withdrawn (WD) for 4 h, or the stimulus withdrawn in the presence of lithium chloride (LiCl) or leptomycin B (LMB) (C) or BAY 11-7082 (BAYi) or MG132 (D) were subjected to western analysis with the indicated antibodies. (E–F) GM-CSF mRNA levels relative to GAPDH were determined in cells treated as indicated. The mean and standard deviation of two independent experiments is shown.