Different colorimetric profiles of ESKAPE pathogens in BacChips.

<p>We loaded devices using a single isolated colony suspended in 100 µL of loading media consisting of 25% LB and 0.05% phenol red in saline. We incubated BacChips at 37°C and imaged the devices after 4 h. Yellow microchambers indicate a decrease in the pH below 6.8, which arises from fermentation of the saccharide; red microchambers match the control and indicate no fermentation (pH 8.2). Orange microchambers indicate that the bacterial are slowly metabolizing the saccharide and the pH is >6.8. Black lines were added to the edge of each device and microchamber to aid in visualization.