Role of the MEK1 MAP kinase pathway in PACAP-induced RAGE shedding.

<p>PAC1/RAGE cells were pre-incubated for 1 h with MEK1 Inhibitor PD98059 (50 µM) and then 300 nM PACAP-27 was added for 2 h. Secreted RAGE was detected and quantified as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041823#pone-0041823-g001" target="_blank">Figure 1</a>. Shown are the mean effects ± S.D., significance was determined by the One-way ANOVA test (ns = P>0.05; ** = P<0.01).