The cross-reactivity among the three markers and their non-cognate antibodies.

<p>They were examined by comparing the fluorescence signals at a definite concentration of CRP (100 mg/L), cTnI (100 ng/mL) and BNP (10 ng/mL) with increasing levels of other two analytes, The five scale points represent the five concentrations of coexistent analytes for each protein respectively. Cross-reactivity among CRP specific antibody and BNP, cTnI was measured after 100 mg/L CRP incubated with specific CRP antibody and different concentrations of BNP and cTnI(with concentration gradient 10, 20, 30, 40, 50 ng/mL). Cross-reactivity among cTnI specific antibody and CRP, BNP was measured after 100 ng/mL cTnI incubated with specific cTnI antibody and different concentrations of CRP and BNP (with concentration gradient 1, 2, 3, 4, 5 mg/L). Cross-reactivity among BNP specific antibody and CRP, cTnI was measured after 10 ng/mL BNP incubated with specific BNP antibody and different concentrations of CRP and cTnI (with concentration gradient 1, 2, 3, 4, 5 mg/L). The number of repeated experiments was five. Error bars represent standard deviations.