The Pax7 lineage outside of the neural crest.

<p>In E15.5 Pax7-cre/reporter embryos, LacZ+ Pax7 descendants are seen in: A) the skin (arrowhead) and skeletal muscle (asterisk); A,B) restricted regions of the carpal bone primordium (arrows) and C) portions of the wholemount gut (arrow). In sections, a subset of LacZ+ cells are detected in: D–F) the eye; the presumptive eyelid muscle (double asterisk), in the lens (arrowhead) and its surrounding tissue (arrow), and neural retina (asterisk); G–I) the lining of the stomach (arrow); J) the pancreas (arrow identifies presumptive acinar cells); K,L) the lining of the gut (arrow; asterisk denotes the liver); and M,N) the skin (arrow), where a distinct border distinguishes the Pax7 lineage from non-lineage Pax7 (arrowhead). O–Q) YFP+ cells visualized with non-fluorescent VIP are detected in: O) the cochlea of the ear; P) lining of lung; and Q) liver (arrows for all). Both YFP and LacZ Pax7-cre/reporters gave the same results, as did fluorescent and non-fluorescent detection methods. Boxes show magnified region in adjacent panel. Scale bars in D,G,K,O–Q are 500 um; E,F,H–J, L–N are 100 um.