Platelet granzyme B apoptosis surveyed by TUNEL in spleen, lung, and kidney.

<p>Representative frozen sections of end organs (i.e. spleen (top), lung (middle), and kidney (bottom)) from wild type (left) and granzyme B null mice (right) were stained for apoptosis with a TUNEL-based assay (TACS® 2 TdT In Situ Apoptosis Detection Kits, Trevigen, Gaithersburg, MD). Increased dark brown staining, evident of apoptosis, is seen in wild type spleens, lungs and kidneys. While the granzyme B null kidneys show apoptosis, there is no staining in the granzyme B null spleens and lungs. No apoptosis was noted in either set of heart and liver sections and is therefore not shown here. Photomicrographs were taken at 10X and 20X magnification. Apoptotic indexes, defined as the number of apoptotic cells per mm², are shown for quantification of tissue apoptosis.