FTIR spectromicroscopy difference spectra of Rat-1 Fibroblasts in lag phase.

<p>FTIR spectra from a cluster of lag phase Rat-1 cells grown on a CaF₂ optical slide. Spectra were recorded (25×25 µm² aperture) over 160 minutes after washing the cells with DMEM prepared with 100% ²H₂O The initial spectrum (defined as t = 0) was used as the reference. The scale is the same as that used in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039685#pone-0039685-g002" target="_blank">Figure 2<i>A</i></a>. The horizontal line indicates that there is essentially no difference between the various spectra.