Immunohistologic analysis for GFP and VEGF expression in the infarcted myocardium after systemic infusion of MNBs/Ad_hVEGF or MNBs/Ad_GFP complexes under magnetic guidance.

<p>(A) Immunohistologic analysis for GFP expression in the infarcted myocardium after 48 h and 7 d treatment. Sections were stained with anti-GFP antibody (green) and nuclei were stained with TOPRO3 (red). (B) Sections from the MI-M+MNBs/AdhVEGF group showed several AdhVEGF transduced cells staining positive interspersed in the luminal lining of the vessel. Sections were stained with anti-hVEGF antibody (yellow), anti-CD31 and nuclei were stained with TO-PRO3 (blue). (C) Occasionally Ad_hVEGF co-localized with cardiac troponin positive cell. Sections were stained with anti-hVEGF antibody (yellow) and anti troponin T antibody (red). Nuclei were stained with TOPRO3 (red). (MI-M⁺MNBs/Ad_GFP, n = 4; MI-M⁻MNBs/Ad_GFP, n = 4).