Exposure to 2 hours of hypoxia does not affect the viability of hippocampal neurons.

<p><b>A.</b> Images of the mouse hippocampal primary culture in control conditions (top) and after 2 hours exposure to 3% O₂ (bottom), at 11 (left) and 18 DIV (right). Cell bodies, axons and dendrites morphology were not affected by the reduction of oxygen supply. <b>B.</b> Bar histogram showing the mean number of cells/mm², counted before and after the hypoxic treatment by the trypan blue exclusion assay. The number of vital cells did not significantly change after hypoxia, either at 11 DIV (left) or 18 DIV (right) (n = 4 dishes, p>0.05, using Student’s paired t-test).