Representative, confocal images of an enriched peripheral blood samples from a SCCHN patient.

<p>Each row of images correspond to a different, confocal, z-slice: 1.38 microns above the middle plane, the middle plane (relative to cell center), and 1.38 microns below the middle plane. A total of 18 z- slices were made, and no indication of CD45 or EpCAM presence was observed in any of the slices. For this set of images, custom conjugated anti-cytokeratin AF 488, anti-CD45 AF594, and a two set, rabbit, anti-EpCAM and anti-rabbit, AF647 was used for staining.