Expression of IL-1beta, and HMGB1 associated with Th17 cell at 1 and 3 month after transplantation compared to before transplantation.

<p>PBMC from patients before KT, patients at 1month after KT and patients at 3 month after KT were stimulated for 4 h ex vivo with PMA and ionomycin in the presence of GolgiStop. PBMC from all groups were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042011#pone-0042011-g001" target="_blank">Figure 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042011#s2" target="_blank">Materials and Methods</a>. The expression of IL-1beta (A), HMGB1(B) mRNA was measured using real-time PCR. Bars show the means.