RNA Polymerase II binding and expression of genes at the <i>sd</i> gene locus during <i>Drosophila</i> development before and after insertion of the <i>Fab-7</i> containing transgene.

<p>(A–C) qRT PCR analysis of <i>sd</i>-RD, <i>CG8509</i> and <i>sd</i>-RE gene expression during <i>Drosophila</i> development in WT, Fab-X and Fab-X,<i>Fab-7</i>¹ flies. RNA was extracted from staged embryos 2 hours (A) 12 hours (B) after egg laying or from female adult flies (C). RNA levels were normalized to the housekeeping gene <i>Rp49</i>. The standard deviation was calculated from at least two independent experiments. Note that primers amplifying the <i>sd</i>-RE transcript also amplify the <i>sd</i>-RD transcript. (D) qChIP analysis of female adult flies in the indicated fly lines using RNA Pol II antibodies. Immunoprecipitated DNA was analyzed by quantitative PCR using primers amplifying genomic regions at the <i>sd</i> gene locus or the <i>Rp49</i> gene. Annotated genes are shown below the graph and are drawn to scale. ChIP signal levels are represented as percentage of input chromatin precipitated for each region. The standard deviation was calculated from at least two independent experiments. (*P<0.05 as calculated from a two-tailed t-test).