Thetiming of ubiquitination is dependent of cell soma size in R6/2 neurons.

<p>Regardless of whether or not huntingtin aggregates (visualized by staining with MW8 and ubiquitin antibodies) formed early or late, aggregates that formed in cells with small (DG neurons) and medium-sized (CA1 and STR) soma were found ubiquitinated within 2 to 3 days of initial aggregation. By contrast, there was typically a more than 3 days lag between initial aggregate formation and inclusion ubiquitination in cells with large soma (CA3). Scale bar = 50 µm.