Analyze of the purified hpMSCs population.

<p>A. The phenotype characteristics of purified MSCs were verified by flow cytometry analysis, which showed that these expanded and plate-adhering populations of cells (at doublings 3 and 5) were positive for CD29 (>95%), CD44 (>95%), CD73 (>99%), CD90 (>95%), CD166 (>95%) and CD105 (>95%) surface marker expression and negative for CD34 (<3%) and CD45 (<3%). The solid line denotes unstained control cells and dotted line denotes isolated mesenchymal stem cells. B. Osteogenic and adipogenic differentiation determination in isolated mesenchymal stem cells. Left panel indicated positive calcium deposit (arrows), right panel indicated lipid vacuoles (arrows). C. Osteogenic and adipogenic differentiation determination in isolated mesenchymal stem cells after transfected with adenovirus. 48 h after transfection, cells were washed with PBS and cultured in OsteoDiff or Adipodiff induction medium for 3 weeks, then Oil-Red-O and Alizarin Red S were utilized to visualize calcium deposits and fat droplets separately. Lipid vacuoles and calcium deposits (arrows) could be visualized (×100).