Transplantation of ‘RSCs’ into adult mouse retina.

<p>Immunohistochemical analysis on adult wild-type retinas transplanted with GFP-positive ‘RSCs’ (green in A, B, C, and D) revealed that the majority of integrated donor cells expressed GFAP, indicative for extensive glial differentiation (A, arrows). While some transplanted ‘RSCs’ differentiated along the neuronal lineage and expressed β-III-tubulin (B, arrows), grafted cells did not show recoverin (C) or rhodopsin (D) immunoreactivity. Also following transplantation into the degenerative retina of rho−/− mice (E) donor ‘RSCs’ (green) did not show immunoreactivity for recoverin (red). Scale bars: 50 µm. Abbreviations: DAPI, 4,6-diamidino-2-phenylindole; GCL, ganglion cell layer; GFAP, glial fibrillary acidic protein; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer.