Knockdown of aldolase B prevented protein and DNA oxidation in high glucose-treated EA. hy926 cells.

<p>Levels of protein carbonyls (A) in cells treated with exogenous MG (**<i>P</i><0.01 vs. control), (B) in cells treated with glucose in the presence or absence of aminoguanidine (AG, 1 mM) and alagebrium (ALA, 100 µM) (*<i>P</i><0.05, **<i>P</i><0.01 vs. 5 mM glucose;^##<i>P</i><0.01 vs. 25 mM glucose), and (C) in cells transfected with control or aldolase B siRNA, or only transfection agent (mock) (**<i>P</i><0.01 vs. 5 mM glucose; ^##<i>P</i><0.01 vs. control siRNA). Levels of 8-oxo-dG (D) in cells treated with exogenous MG (**<i>P</i><0.01 vs. control), (E) in cells treated with glucose in the presence or absence of aminoguanidine (AG, 1 mM) and alagebrium (ALA, 100 µM) (*<i>P</i><0.05, **<i>P</i><0.01 vs. 5 mM glucose;^##<i>P</i><0.01 vs. 25 mM glucose), and (F) in cells transfected with control or aldolase B siRNA, or only transfection agent (mock) (**<i>P</i><0.01 vs. 5 mM glucose; ^##<i>P</i><0.01 vs. control siRNA). Fluorescence intensity of 8-oxo-dG per cell was measured using Image J.