AbM extract induced caspase-1 activation in THP-1 macrophages.

<p>(<b>A</b>) and (<b>C</b>) Procaspase-1 p45 and caspase-1 subunit p20 were detected in cell lysates by Western blot analysis. (<b>B</b>) The secretion of the caspase-1 subunit p20 into the supernatants of THP-1 cells treated with AbM at concentrations of 0.1–5% for 24 h was assessed by ELISA. (<b>D</b>) THP-1 macrophages were untreated or pretreated for 30 min with the caspase-1 inhibitor, Z-YVAD-fmk (20 µM). Subsequently, cells were incubated with AbM at concentrations of 0.1–5% for 24 h, and IL-1β production was measured by ELISA. Data are presented as means ± SE of 3 experiments preformed in duplicate. *<i>P</i><0.05 versus AbM-untreated control cells. ^#<i>P</i><0.05 versus caspase-1 inhibitor-treated cells.