Knockdown of aldolase B prevented high glucose-increased NF-κB nuclear translocation in EA. hy926 cells.

<p>(A) Nuclear p65 subunit of NF-κB in cells treated with exogenous MG for 3 days. **<i>P</i><0.01 <i>vs</i>. control (5 mM glucose). (B) Nuclear p65 subunit of NF-κB in cells treated with glucose (Glu) in the presence or absence of aminoguanidine (AG, 1 mM) and alagebrium (ALA, 100 µM). **<i>P</i><0.01 <i>vs</i>. 5 mM glucose;^##<i>P</i><0.01 <i>vs</i>. 25 mM glucose. (C) Nuclear p65 subunit of NF-κB in cells transfected with control or aldolase B siRNA, or only transfection agent (mock). **<i>P</i><0.01 <i>vs</i>. 5 mM glucose; ^##<i>P</i><0.01 <i>vs</i>. control siRNA.