Cross-linking results for FGFR3/WT and FGFR3/A391, under conditions which allow visual comparison between the wild-type and the mutant.

<p>2.5 µg and 4 µg of wild-type and mutant DNA were used for transfection in these experiments, in order to achieve similar expression. The lysate loading was the same in the numbered lanes, which were used for quantification. The mutation induces a modest, but statistically significant increase in cross-linked fraction from 0.28±0.03 to 0.37±0.05 (p = 0.03). The cross-linking results under the transfection conditions used in the activation studies are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056521#pone-0056521-t001" target="_blank">Table 1</a>.