Seed-germination phenotypes of 35S::AaPYL9 and 35S::AaPYL9-H116A transgenic <i>Arabidopsis</i> plants.

<p>A: ABA-hypersensitive inhibition of germination in 35S::AaPYL9 and 35S::AaPYL9-H116A transgenic <i>Arabidopsis</i> lines compared with wild-type plants and ABA receptor triple mutant. Seeds were grown on MS medium with or without 0.3 µM ABA or 0.5 µM ABA. Photographs were taken 8 days after sowing. B: Percentage of seed germination and early seedling growth in the presence of the indicated ABA concentrations are shown. Germination percentage is determined at 8 days after seeds scored on MS (black bars), 0.3 µM ABA (dark gray bars) or 0.5 µM ABA (white bars). Data are averages ±SE from three independent experiments (n≈200 seeds per experiment). *P<0.01 (Student's t test) when comparing data for each genotype versus the wild-type under the same conditions. C: RT-PCR analysis of <i>AaPYL9</i> expression in 35S::AaPYL9 and 35S::AaPYL9-H116A transgenic <i>Arabidopsis</i> lines. RT-PCR was carried out for 30 cycles, β<i>-ACTIN8</i> transcripts were amplified as a control (lower gel).