Neither the IRF3 nor NFκB singaling pathway appears to be involved in decreased IFN-α responses induced by simvastatin.

<p>mRNA expression levels of IRF3 (<b>A</b>) and NFκB (<b>B</b>) in simvastatin-treated macrophages at 9 and 24 hours after poly (I:C) stimulation. The mRNA expression levels were measured by qRT-PCR and normalized to the expression levels of β-actin. Each bar represents the mean ± SEM. Data from 2 independent experiments were combined, and each test was performed in triplicate.