Genetic interactions between <i>zig-5, zig-8</i> and <i>sax-7</i>.

<p>(A) ASI and ASH neuronal displacements in mutant adult animals scored with the <i>oyIs14</i> reporter transgene. Wild-type and <i>zig-5 zig-8</i> double mutant pictures are the same as shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002819#pgen-1002819-g001" target="_blank">Figure 1C</a> and shown for comparison only. See legend to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002819#pgen-1002819-g001" target="_blank">Figure <i>1C</i></a> for explanation of symbols. <i>Scale</i> bar is 5 µm. (B) Quantification of ASI and ASH neuronal position in genetic different backgrounds. “punc-14” is a panneuronal driver. <i>Punc-14::sax-7L</i>: DNA was injected at 75 ng/uL (line 1) or 50 ng/uL (lines 2 and 3). <i>Punc-14::sax-7S and Punc-14::sax7Δ11</i>: DNA was injected at 50 ng/uL. Proportions of different animal populations were compared using the z-test. “*” indicates p<0.001. (C) Genetic interactions of <i>zig-5, zig-8</i> and <i>sax-7</i> in controlling axon positioning in the ventral nerve cord. Quantification of PVQ axon flip-overs with transgene <i>oyIs14</i>. Error bars indicate s.e.p. Proportions of different animal populations were compared using the z-test. “*” indicates p<0.01. (D) Effect of ectopic expression of various forms of <i>sax-7</i> in a wild-type background. ASI and ASH neuronal position are quantified. Proportions of different animal populations were compared using the z-test. “*” indicates p<0.001. (E) Quantification of ASI and ASH neuronal position in <i>dig-1(ky188)</i> mutant animals. “<i>Punc-14</i>” is a panneuronal driver for expression of SAX-7S. There are no statistically significant differences between <i>dig-1</i> animals and any of the transgenic <i>dig-1</i> animals expressing <i>sax-7S.</i>