<i>Mgat1</i> mutants successfully initiate capsule formation but have a failure in lamellocyte consolidation.

<p>Brightfield and fluorescent images of <i>L. clavipes</i> eggs dissected from attacked fly larvae. (A,B) Plasmatocytes are marked by the expression of <i>eater-GFP</i> in <i>w¹¹¹⁸</i> (A) and <i>Mgat1¹/+</i> (B) backgrounds. At 0–24 hours PA, plasmatocytes in both genetic backgrounds migrated to, and surrounded, the wasp egg. (C–H) Lamellocytes are marked by the expression of <i>msn-mCherry</i>. At 24–48 hours PA, lamellocytes from both <i>w¹¹¹⁸</i> (C) and <i>Mgat1¹/+</i> (D) surrounded the wasp egg. By 48–72 hours PA, <i>w¹¹¹⁸</i> larvae formed a consolidated lamellocyte layer around the wasp egg, indicated by arrows (brightfield in E, <i>mCherry</i> fluorescence in F). At the same time point, this consolidated layer was not seen in <i>Mgat1¹/+</i> larvae, and instead individual lamellocytes were still visible (brightfield in G, <i>mCherry</i> fluorescence in H). (I) Additional brightfield image of a wasp egg from a <i>Mgat1¹/+</i> larva that lacked a consolidated lamellocyte layer but was surrounded by individual lamellocytes. (J,K) Close up images of panels E and I (respectively) to focus on outer layer morphology of eggs at the 48–72 hour PA time point. A consolidated layer was consistently observed in <i>w¹¹¹⁸</i> larvae (indicated by arrow in J) whereas in <i>Mgat1¹/+</i> larvae (K), individual lamellocytes were clearly seen. Scale bars indicate 100 µm in A–I and 50 µm in J,K.