Identification of gene probes differentially expressed between the cecum (including ileocecal valve and appendiceal orifice) and the sigmoid colon by two-way SAM analysis.

<p>A) 347 gene probes were significantly elevated in the cecum relative to the sigmoid colon, 47 of which were involved in immunologic processes as determined by gene ontology analysis. 160 gene probes were significantly enriched in the sigmoid colon compared to the cecum, 35 of which were involved in immunologic processes. Genes probes related to immunologic processes are shown in the table below the figure. Analyses shown were performed at a FDR of 0%. B) Verification of gene expression by real-time PCR analysis of PPARA, PPARG, and MUC1 normalized to GAPDH transcript levels in 40 intestinal biopsy samples. Values were log transformed as shown. Horizontal bars indicate median expression levels. Statistical significance between groups was determined by two-tailed Mann Whitney tests. *<i>P</i><0.05; **<i>P</i><0.01; ***<i>P</i><0.001.