AMPK inhibition by Compound C causes spindle misorientation and other mitotic defects in MDCK cell cysts.

<p>A) Representative spindles from vehicle treated control cysts. Images were rotated in three dimensions to show the cyst lumen, place the apical cell surface at the top of the cell, and place both spindle poles in a single plane. Spindle angle was measured relative to the apical surface. Microtubules are green, actin is red, and DNA is blue. B–D) Representative spindles from cysts treated with 20 µM Compound C for 4 hours prior to fixation. Images in B are displayed as in A. Images in C and D are taken from single lumen cysts, although the lumen is not visible in every image. Insets in D show magnified cells with misattached chromosomes marked by white arrows. Scale bar, 10 µm. E) Quantification of spindle angles. Each dot represents a single spindle angle measurement. Blue bars represent means and standard error of the mean. See text for numbers. P<0.0001 for the difference. F) Data from (E) presented as the % of angles that range from 0° to 30°, 30° to 60°, and 60° to 90°.