Expressions of ER stress-related molecules GRP78 and CHOP in the mouse hippocampus with or without tunicamycin treatment.

<p>C57BL/6J mice were intracerebroventricular injected with tunicamycin (0.02–1 mg/ml) at various time course (6–72 h). In A, the levels of GRP78 and CHOP proteins in hippocampus were determined by Western blotting 24 h after tunicamycin injection in a dose-dependent manner. Representative images of three independent experiments are shown. In B, the levels of GRP78 and CHOP proteins from hippocampus were determined by Western blotting after tunicamycin (0.1 mg/ml) injection in a dose-dependent manner. In C, the levels of GRP78 and CHOP mRNAs from hippocampus were determined by real-time PCR after tunicamycin (0.1 mg/ml) injection in a time-dependent manner. In B and C, quantification of proteins and mRNA levels were shown. Data are presented as mean ± S.E.M. (n = 3). *P<0.05 as compared with sham-control.