MDSC depletion augmented the tumor cell apoptosis and increased anti-angiogenic but reduced pro-angiogenic marker expression in tumors.

<p><b><i>4A</i></b><i>,</i> IHC for cleaved caspase 3 in tumor sections following MDSC depletion. The arrows are indicative of cleaved caspase 3 staining. <b><i>4B</i></b><i>,</i> AnnexinV and PI staining in tumor digests by flow cytometry. <b><i>4C</i></b><i>,</i> Relative caspase 8 expression by QRTPCR normalized with β-actin expression in tumor tissues. <b><i>4D</i></b><i>,</i> Endothelial MECA 32 cell marker expression and total T cell CXCR3 expression in tumors by flow cytometry. <b><i>4E–H</i></b><i>,</i> Relative pro-angiogenic and anti-angiogenic marker expression in tumors by QRTPCR normalized by β-actin. MDSC depletion decreased pro-angiogenic (VEGF-a, CXCL2, CXCL5, Ang1 and Ang2) (<b>4E–F</b>) but increased anti-angiogenic (CXCL9 and CXCL10) (<b>4G</b>) and CXCR3 (<b>4H</b>) expression in the tumors. (Data, mean ± SEM, p values: compared to controls *p<0.05, n = 8 mice/group).