L-NAME inhibits simulated microgravity-induced HUVEC-C migration.

<p>An in vitro wound-healing assay was performed to evaluate the migration abilities of HUVEC-C treated with clinorotation in the absence or presence of L-NAME. The confluent HUVEC-C were wounded by scraping with a yellow tip. The cells were (A, B, C) washed, and then (D, E, F) incubated 24 h with fresh medium. The migration ability was determined by measuring the width of each denuded area and by calculating through the formula: 100%−(width _{24 h}/width_{0 h}) × 100%. (G): Result depicted that simulated microgravity caused an obviously increase in wound healing which was abrogated by L-NAME treatment. The data represent mean ± SD of three independent experiments. ^*<i>P</i><0.05 versus control group; ^#<i>P</i><0.05 versus clinorotation group.