Surfactant lipid component and its uptake by alveolar monocytes.

<p>(A) Saturated phosphatidylcholine (Sat PC µmol/kg) in CHF5633 (Input), Survanta (Input), lamb lung, and BALF were analyzed. Two fold higher Sat PC was instilled to CHF5633 group than Survanta group and Sat PC in the lung was higher for CHF5633 group than Survanta group. *p<0.001 vs. Survanta In contrast, Sat PC in BALF was similar between the 2 groups, suggesting increased CHF5633 catabolism compared with Survanta. (B) Microphotograph of alveolar cells. Phagocytosis of surfactant by monocytes was higher for the CHF5633 group and monocytes contained lipid droplets. Scale bar: 10 µm. (C) Phosphatidylcholine (PC) and phosphatidylglycerol (PG) were analyzed by thin layer chromatography. There were similar amounts of PC and PG in CHF5633 and the major component of Survanta was PC. The ratio of PC to PG did not change in the ventilated preterm lamb lung and was similar to Input samples.