Bafilomycin A increased the level of LC3B-II in cytotrophoblasts under hypoxia.

<p>Cytotrophoblasts were incubated under standard or hypoxic (2% oxygen) conditions with or without bafilomycin A1, an inhibitor that inhibits degradation of autophagosome content. Treatment with bafilomycin A1 increased LC3B-II, indicating that these cells had a high basal rate of autophagy under hypoxia. Horizontal bars represent the median values. <i>P</i> values were based on the Kruskal-Wallis test followed by Dunn’s multiple comparison test. A total of 10 individual experiments were performed.