Tocopherol treatment of TNFα-stimulated HMVECLs was not cytotoxic and did not alter ICAM-1 expression.

<p>70% confluent monolayers of HMVECLs were pretreated for 6 hrs with TNFα (10 ng/ml) to induce ICAM-1 expression. Then, the endothelial cells were treated for 16 hrs with α-tocopherol (α-toc) (40, 60 or 80 µM), γ-tocopherol (γ-toc) (1, 2 or 4 µM) or the vehicle control DMSO (0.01%). The cells were examined for cytotoxicity with the <b>A</b>) Vybrant Cytotoxicity Assay or examined by <b>B,C</b>) immunolabeling with FITC-labeled anti-ICAM-1 and flow cytometry. Shown are the means ± SEM from 3 experiments. MFI, mean fluorescence intensity. *, p<0.05 compared with non-treated (NT) cells.