Representative images of morphological features in the retinal layer following laser burn injury in B6 mice.

<p>Tissue were embedded in paraffin and stained with hematoxylin and eosin. Eye receiving sham laser injury shows intact retina and RPE layers (A). At 12–24 hr after the laser burns, INL and ONL begin to show structural disorganization with some photoreceptors loss (B – C). RPE monolayer was disrupted and pigmented cells were observed in the subretinal space (red arrow; C). On day 3, significant photoreceptors loss was observed in conjunction within the laser-injured area (D). No photoreceptor was found in the injury area at day 14 (E, indicated by blue arrows). The RPE monolayer reformed at the wounded area suggesting reformation of a new blood-retina barrier (F, indicated by red arrows). The scale bar for all images: 100 µm. Abbreviation: GCL, ganglion cell layer.