IFN-γ potentiates the GM-CSF priming effects on BMDM.

<p>BMDM WT cells were preincubated for 24 h with GM-CSF or GM-CSF plus IFN-γ (500 UI/mL) and then stimulated with AraLAM (5000 ng/mL), LM (5000 ng/mL), Malp2 (300 ng/mL), Pam₃-CSK₄ (5000 ng/mL) or LPS (500 ng/mL) for 24 h <i>in vitro</i>. The supernatants were analyzed for the production of NO (Nitrite) (<b>A</b>), TNF-α (<b>B</b>), PGE₂ (<b>C</b>) and IL-10 (<b>D</b>). Medium alone was used as a negative control. ELISA was used to measure TNF-α and PGE₂, and the Griess Method was used to measure Nitrite production in the supernatants. Results are the average ± S.E.M from mice cells (<i>n</i> = 6 per group) from two independent experiments; *, <i>p</i><0.05 compared with unstimulated cells; <b>^#</b>, <i>p<0.05</i> compared with GM-CSF priming alone.