Percentage of fungal taxa amplified <i>in silico</i>.

<p>One nucleotide mismatch between each target primer and the database sequence was allowed in ecoPCR, except for the two bases at the 3′-end of the primer.</p>*<p>ITS2, used to construct subset 1, was used as a reverse primer in ecoPCR.</p>†<p>ITS5, used to construct subset 2, was used as a forward primer in ecoPCR.</p>‡<p>ITS4, used to construct subset 2, was used as a reverse primer in ecoPCR.</p>§<p>ITS3, used to construct subset 3, was used as a forward primer in ecoPCR.