c-Met, p-Met and RPE65 expression in c-Met^fl/fl mice 14 days after AAV-GFP and AAV-Cre injections, respectively (A, C, E, G, I and K) without laser burns; and after laser burns (B, D, F, H, J and L).

<p>Mice were scarified on day 14 after laser application (total day, 28). In AAV-GFP injected mice, laser burns induced an expected increase in c-Met and p-Met (B vs. A; E vs. F). There was no detectable c-Met or p-Met expression was seen after subretinal AAV-Cre injection (C and G). Less c-Met and no p-Met expressed were observed even after laser injury (D and H). RPE65 expression was not affected by subretinal injection of AAV-GFP or AAV-Cre injection (I – L). There were more migrated RPE cells in ONL in AAV-Cre injected (L) compared to AAV-GFP injected mice (J). There was very limited c-Met and p-Met detected in AAV-Cre injected eyes before and after laser injury (SS1– SS2); in AAV-GFP injected eyes, c-Met expression expectedly increased after laser treatment (SS1) (* P<0.05, independent samples <i>t</i>-test). RPE65 expression was not affected by either AAV-GFP or AAV-Cre injection (SS3).