Transfection with LHR1 increases the intracellular heme content of <i>L. amazonensis</i> promastigotes.

<p>(A) Absorption spectra of the hemochrome content of lysates of increasing numbers of <i>L. amazonensis</i> promastigotes transfected with vector alone or with <i>LHR1-3xFLAG</i>, and grown in heme-containing medium. (B) Heme concentrations in (A) calculated based on the heme millimolar extinction coefficient of 20.7. The data represents the mean+/−SD of triplicate determinations. p = 0.199 (1×10⁹), ** p = 0.001 (2×10⁹), * p = 0.054 (4×10⁹) (two-tailed Student's t test).