Anti-PDL-1 antibody treatment increases the proportion of IL-2, IFN-γ and TNF-α producing SSIEFARL specific effector CD8 T cells.

<p>Mice from anti-PDL-1 or isotype treated groups were euthanized on day 6 post infection. Single cell suspensions obtained from PLN and spleen tissue were stained intra-cellularly for pro-inflammatory cytokines after brief <i>in vitro</i> stimulation with SSIEFARL peptide (10 µg/ml). The representative FACS plots in A, B and C demonstrates the proportion of IFN-γ, TNF-α and IL-2 secreting CD8 T cells, respectively, in PLN and spleen tissue derived from isotype and anti-PDL-1 treated mouse. Scatter plots in panel A, B and C show mouse to mouse variation in the frequency of IFN-γ, TNF-α and IL-2 producing CD8 T cell in PLN and spleen tissue of isotype and anti-PDL-1 treated groups of mice. Data shown is derived from two similar experiments with four mice per group in each experiment. Statistical significance was determined by unpaired student’s t test where *p<0.05 and **p<0.01 are considered statistically significant.