RT-PCR for detecting AMP gene transcripts during development and after LPS treatment.

<p>Expression of, <i>Diptericin (dipt), drosocin (drc), cecropinA (cecA) and attacinA (attA),</i> in MT from 1^st, 2^nd, 3^rd, prepupae, pupae and adult under normal conditions (A). The intensity of bands were measured and plotted on a graph (A’). <i>AttacinA</i> and <i>Drosocin</i> is highest in adult, whereas <i>diptericin</i> and <i>cecropinA</i> is highest in pupae. MT from 3^rd instar, prepupae, pupae and adult were treated with LPS and RT-PCR was done (B). Enhanced expression of all the AMP were observed after LPS treatment (LPS) and compared with control (C) without LPS treatment. The intensity of bands were measured and plotted on a graph (B’) which show that after immune challenge there is an enhanced expression of all AMP at all stages of development. <i>Glyceraldehyde-3-phosphate dehydrogenase (GPDH)</i> is used as an internal control to ensure the integrity of RT-PCR.