A. <i>E. coli-GFP</i> expression in gut of larvae.

<p>No fluorescence is observed in the gut of control larvae, not fed on GFP bacteria of 1^st instar (A) 2^nd instar (B) and 3^rd instar (D). A’, B’ and C’ are DIC images merged with fluorescence shows that the gut region is devoid of GFP-bacteria. Green fluorescent is observed when 1^st (D), 2^nd (E) and 3^rd (F) instar larvae are fed on GFP-bacteria. DIC images and merged fluorescence (D’, E’ and F’) confirms that green fluorescence is in the gut. <b>B</b>. <b>Mortality rate of different stage larvae when fed on bacterial food.</b> Percentage of adults emerged shows that 1^st instar (green graph) wild type larvae are most susceptible to <i>E. coli</i> and <i>M. smegmatis</i> than 2^nd instar (yellow graph) and 3^rd instar (maroon graph). <i>imd</i> mutants (control) are less viable than <i>diap2</i> mutants (control) and also the mortality rate for <i>imd</i> mutants are significantly less than <i>diap2</i> mutant when fed on <i>E. coli.</i> Asterick (*) represents significance at p<0.05.