Effects of topical LT or IL-18 on RBC velocities in mesentery capillaries.

<p>Dashed vertical lines represents initial application of topical agent (prior to agent is topical saline). Points represent the average velocity of RBCs within the three areas viewed at the indicated times (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040126#s4" target="_blank">Methods</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040126#pone-0040126-g002" target="_blank">Fig 2</a> for details). Due to the change in slopes over time, two regression lines were calculated. The first regression line uses the first four viewing intervals. The second line uses the fourth through seventh viewing intervals. Error bars represent standard error of the mean. <b>A</b>) The slope of regression line 1 (asterisk), but not regression line 2, for RBC velocities in capillaries was more negative in B6.CAST.11M mice given topical application of LT compared to C57BL/6J mice given LT (p = 0.008). B6.CAST.11M LT group n = 8, and B6.CAST.11M saline and C57BL/6J LT groups n = 5. <b>B</b>) B6.CAST.11M and C57BL/6J mice given topical application of IL-18. RBC velocities in capillaries remained relatively unchanged over time and there were no differences for RBC velocities between B6.CAST.11M and C57BL/6J mice given topical application of IL-18. Both animal groups, n = 5. <b>C</b>) The area under the curve (corresponding to panel A) was less in B6.CAST.11M mice given topical application of LT compared to C57BL/6J mice given topical application of LT (p = 0.006) (# indicates arbitrary units). <b>D</b>) The area under the curve (corresponding to panel B) was comparable between C57BL/6J and B6.CAST.11M mice given topical application of IL-18.