<i>Mw</i> induced Th1 response and NO generation in <i>L. donovani</i> infected BALB/c mice (A–F).

<p>Splenocytes from infected and differently–treated mice, as mentioned in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040265#pone-0040265-g006" target="_blank">figure 6</a> legend, were isolated on 56 days after infection, plated aseptically (2×10⁵ cells/well), and stimulated with 10 µg/ml soluble leishmanial antigen (SLA) for 48 h. Interferon γ (IFN- γ) (A), nitric oxide (B), Tumor necrosis factor α (TNF-α) (C), Interleukin 10 (IL-10) (D) Interleukin 12 (IL-12) (E) and Tumor growth factor β (TGF- β) (F) from the culture supernatants of the indicated treatment groups were determined by enzyme-linked immunosorbent assay and the Nitric Oxide Colorimetric Assay kit, respectively. Real-Time polymerase chain reaction (PCR) analysis was performed from similar experimental sets to determine proinflammatory (IFN-γ, IL-12p70, TNF-α), anti-inflammatory cytokines (IL-10, TGF-β) and iNOS2 mRNA expression (G, H). Results are from three independent experiments. Data are means ± standard deviations of values from 3 independent experiments that yielded similar results^*P<0.001, ^**P<0.01 compared with infected mice and ^***P<0.05 compared with <i>Mw</i> treated infected mice; paired two-tailed Student’s t-test.